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1.
Accid Anal Prev ; 201: 107569, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38615505

RESUMO

BACKGROUND: Globally, road traffic crashes are the leading cause of death for young adults. The P Drivers Project was a trial of a behavioural change program developed for, and targeted at, young Australian drivers in their initial months of solo driving when crash risk is at its highest. METHODS: In a parallel group randomised controlled trial, drivers (N = 35,109) were recruited within 100 days of obtaining their probationary licence (allowing them to drive unaccompanied) and randomised to an intervention or control group. The intervention was a 3 to 6-week multi-stage driving behaviour change program (P Drivers Program). Surveys were administered at three time points (pre-Program, approximately one month post-Program and at 12 months after). The outcome evaluation employed an on-treatment analysis comprising the 2,419 intervention and 2,810 control participants who completed all required activities, comparing self-reported crashes and police-reported casualty crashes (primary outcome), infringements, self-reported attitudes and behaviours (secondary outcomes) between groups. RESULTS: The P Drivers Program improved awareness of crash risk factors and intentions to drive more safely, relative to the controls; effects were maintained after 12-months. However, the Program did not reduce self-reported crashes or police-reported casualty crashes. In addition, self-reported violations, errors and risky driving behaviours increased in the intervention group compared to the control group as did recorded traffic infringements. This suggests that despite the Program increasing awareness of risky behaviour in novice drivers, behaviour did not improve. This reinforces the need to collect objective measures to accompany self-reported behaviour and intentions. CONCLUSIONS: The P Drivers Program was successful in improving attitudes toward driving safety but the negative impact on behaviour, lack of effect on crashes, and the large loss to follow-up fail to support the use of a post-licensing behaviour change program to improve novice driver behaviour and reduce crashes. TRIAL REGISTRATION: Australian New Zealand Clinical Trials Registry: 363,293 (ANZCTR, 2012).


Assuntos
Acidentes de Trânsito , Condução de Veículo , Humanos , Condução de Veículo/psicologia , Condução de Veículo/educação , Acidentes de Trânsito/prevenção & controle , Masculino , Feminino , Adulto Jovem , Austrália , Adolescente , Adulto , Avaliação de Programas e Projetos de Saúde , Intenção , Segurança , Assunção de Riscos , Fatores de Risco , Conhecimentos, Atitudes e Prática em Saúde
2.
Accid Anal Prev ; 146: 105750, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32932019

RESUMO

While cyclist aggression is relatively rare, it has been associated with increased crash risk. Previous measures of cyclist aggression have not taken into consideration who the recipient of the aggression may be; this is likely to differ across road user types. The aim of this study was to understand if cyclists' aggression differs according to whether the recipient is a driver, pedestrian or another cyclist. To examine this, 1206 cyclists (males = 75 %; age range 18-80 years; M = 47.35 ± 11.81) completed the Cycling Anger Expression Inventory (CAX; Møller and Haustein, 2017) three times; once for each road user type. Respondents also provided information regarding their cycling anger tendencies. Open text responses regarding sources of, and responses to, anger were also sought. The measurement invariance of the three CAX models was examined to determine whether the items were interpreted in a similar manner for interactions with each road user type and to compare latent factor means. The results showed that verbal aggression was not the same across road users. However, adaptive constructive ways of dealing with anger were similar across the three types of recipients. Comparison of latent means showed that cyclists reported higher expressions of anger toward drivers than cyclists or pedestrians. Qualitative analysis of the text responses suggest this is due to the perceived risk posed by drivers combined with positive attitudes expressed towards sharing infrastructure with pedestrians and other cyclists. Based on these findings it was concluded that: i) the CAX might best be used with reference to drivers, rather than "road users", and ii) while aggression in cyclists is rare, it is more common toward drivers than other road users.


Assuntos
Agressão/psicologia , Ira , Ciclismo/psicologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Ciclismo/estatística & dados numéricos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Autorrelato , Adulto Jovem
3.
Accid Anal Prev ; 144: 105625, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32526500

RESUMO

Anger is a common behaviour exhibited by road users when one's goals are perceived to have been blocked by another. Recent research has demonstrated that, generally, cyclists tend to deal with anger in constructive ways. However, when anger does manifest, it can result in behaviours that increase their crash risk. Amongst motor vehicle drivers, mindfulness levels have been associated with less anger and appear to mediate anger and associated aggression. The current study sought to understand whether mindfulness has similar associations with anger and aggression in a sample of cyclists. A total of 583 cyclists (males = 68 %) completed an online questionnaire that sought information on their levels of mindfulness, current mindfulness practices and tendencies for anger and aggression while cycling. The relationships between these were then examined using structural equation modelling. The results showed that cyclists with higher mindfulness levels tended to report less anger across a range of situations (e.g., interactions with pedestrians, cyclists, motor vehicle drivers and police). Both direct and indirect (through anger) relationships were found between mindfulness and aggression, again showing that more mindful cyclists tended to engage in less frequent aggression. These findings align with recent research investigating this relationship amongst motor vehicle drivers and suggest that mindfulness may be a promising strategy to reduce or avoid anger and aggression in cyclists.


Assuntos
Ciclismo/psicologia , Atenção Plena , Fúria no Trânsito/psicologia , Acidentes de Trânsito/prevenção & controle , Adulto , Ciclismo/estatística & dados numéricos , Feminino , Humanos , Análise de Classes Latentes , Masculino , Pessoa de Meia-Idade , Pedestres/estatística & dados numéricos , Fúria no Trânsito/estatística & dados numéricos , Autorrelato , Inquéritos e Questionários
4.
Accid Anal Prev ; 131: 137-145, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31255799

RESUMO

It is well established that angry and, subsequently, aggressive drivers pose a problem for road safety. Over recent years, there has been an increase in the number of published studies examining driver anger, particularly using the Driving Anger Scale (DAS). The DAS measures six broad types of situations likely to provoke anger while driving (i.e., police presence, illegal driving, discourtesy, traffic obstructions, slower drivers, and hostile gestures). The majority of the recent studies have moved away from traditional paper-and-pencil methodologies, using the internet to collect data, for reasons of convenience. However, it is not yet completely clear whether data obtained from this methodology differs from more traditional methods. While research outside of the driving arena has not found substantial differences, it is important to establish whether this also applies to driving-related research and measures, such as the DAS. The present study used Multigroup Confirmatory Factor Analysis (MGCFA) to investigate the invariance of the DAS across a random sample from the electoral roll (n = 1,081: males = 45%) and an internet sourced sample (n = 627; males = 55%). The MGCFA showed the same six-factor solution was supported in both datasets. The relationships between the DAS factors and age, sex, trait anger, and annual mileage were broadly similar, although more significant differences were identified in the internet sample. This research demonstrates that driving measures administered over the internet produce similar results to those obtained using more traditional methods.


Assuntos
Direção Agressiva/psicologia , Ira , Fúria no Trânsito , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Análise Fatorial , Feminino , Humanos , Internet , Masculino , Pessoa de Meia-Idade , Autorrelato , Estatísticas não Paramétricas , Adulto Jovem
5.
Accid Anal Prev ; 125: 124-131, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30743175

RESUMO

There is extensive evidence that using a mobile phone whilst driving is one of the biggest contributors to driver distraction, which in turn increases the risk of motor vehicle collisions. Whilst most of the developed countries have been trying to deter this behaviour through legislation, enforcement and educational campaigns, in Ukraine, where the road fatality rate is the highest in Europe, this issue has only recently become publicised. The present study examined psychological factors that are associated with hand-held mobile phone use while driving among a sample of Ukrainian drivers, in particular writing or reading a text message while driving. This included drivers' behavioural, normative, and control beliefs relating to mobile phone use while driving, as well as the degree to which using a mobile phone is integral to one's everyday life (measured using the Mobile Phone Involvement Questionnaire; MPIQ). Almost one quarter to one third of the sample reported using their phone on a daily basis to write (22.2%) or read (38.2%) text messages while driving. A binary logistic regression showed that gender, higher MPIQ scores, perceived approval from family members, lower perceived likelihood of receiving traffic fines and less demanding traffic conditions were all significantly associated with mobile phone use while driving. These results suggest that dependence upon a mobile phone in everyday life may be an important factor to consider when developing interventions to reduce hand-held mobile phone use while driving.


Assuntos
Uso do Telefone Celular , Telefone Celular , Direção Distraída , Conhecimentos, Atitudes e Prática em Saúde , Envio de Mensagens de Texto , Acidentes de Trânsito , Adulto , Idoso , Condução de Veículo , Europa (Continente) , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Inquéritos e Questionários , Ucrânia , Adulto Jovem
6.
Accid Anal Prev ; 113: 171-178, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29407664

RESUMO

The aim of the current study was to examine how self-reported aberrant driving behaviours change across a three time-points in a group of older drivers. Two hundred and twenty-seven older drivers (males = 69.6%) from the Candrive/Ozcandrive longitudinal study completed the Driving Behaviour Questionnaire (DBQ) each yearacross three time-points (i.e., Year 1, Year 2, Year 3). At the third time-point, older drivers ranged in age from 77 to 96 years (M = 81.74 years; SD = 3.44 years). A longitudinal confirmatory factor analysis showed that a modified 21-item, 3-factor (errors, lapses and violations) DBQ was invariant across the time period, suggesting that the structure of the questionnaire was stable across each time-point. Further, multiple domain latent growth analysis on the resultant factors for errors, lapses and violations showed that the frequency of errors remained similar across the three-year period, while violations and lapses showed very marginal decreases in frequency. These changes were independent of the absolute number of these behaviours; Drivers with higher violations or lapses in Year one, showed similar decreases in frequency as those who self-reported lower frequencies of the behaviours. These results suggest that the DBQ is a reliable tool to measure older drivers' self-reported aberrant driving behaviours, and that these behaviours do not show much change across time. Future research should validate the self-reported responses from the DBQ with more objective measures such as those collected through naturalistic driving study (NDS) methodology or on-road driving tasks.


Assuntos
Acidentes de Trânsito/prevenção & controle , Condução de Veículo/normas , Inquéritos e Questionários , Idoso , Idoso de 80 Anos ou mais , Comportamento , Análise Fatorial , Feminino , Avaliação Geriátrica , Humanos , Estudos Longitudinais , Masculino , Reprodutibilidade dos Testes , Autorrelato
7.
Accid Anal Prev ; 106: 23-30, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28550751

RESUMO

The present study investigated the validity of the 25-item Driving Anger Expression Inventory (DAX) as well as the role of sex and gender-roles in relation to the expression of driving anger in a sample of 378 French drivers (males=38%, M=32.9years old). Confirmatory Factor Analysis supported the four-factor structure of the 25-item DAX (Adaptive/Constructive Expression; Use of the Vehicle to Express Anger; Verbal Aggressive Expression and Personal Physical Aggressive Expression) and two of the three aggressive factors were found to have significant positive relationships with driving anger, while adaptive/constructive expression was negatively related to driving anger. Use of the vehicle to express anger was not significantly related to crash involvement, but was significantly related to all other crash-related conditions (traffic tickets, loss of concentration, loss of control of the vehicle, near crash). The presence of feminine traits, but not sex, was predictive of adaptive/constructive behaviours, while masculine traits predicted more frequent verbal aggressive expression, use of the vehicle to express anger, personal physical aggressive expression and total aggressive expression. This finding may account for the inconsistent relationship found between driving anger and sex in previous research. This research also found that the 25-item DAX is a valid tool to measure the expression of driving anger and that the endorsement of masculine traits are related to more aggressive forms of driving anger expression.


Assuntos
Agressão/psicologia , Ira , Condução de Veículo/psicologia , Feminilidade , Masculinidade , Acidentes de Trânsito/psicologia , Acidentes de Trânsito/estatística & dados numéricos , Adaptação Psicológica , Adulto , Condução de Veículo/estatística & dados numéricos , Análise Fatorial , Feminino , França , Humanos , Masculino , Fatores Sexuais , Inquéritos e Questionários
8.
Accid Anal Prev ; 103: 56-64, 2017 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28384489

RESUMO

BACKGROUND: Vehicle speed is a major contributor to road trauma, both in terms of increased crash risk and injury severity. In Australia, approximately one third of fatal crashes occur in speed zones of 100km/h. This proportion has remained the same, despite the reduction in the number of road fatalities over the past decade. To drive further reductions in speed-related crashes, an improved understanding of the underlying determinants of speed choice is required. METHOD: A community attitude survey designed to understand speed behaviour and attitudes towards speeding was distributed to a large (N=5179) representative sample of drivers in Australia. Participants provided information regarding their normal speed choices across four different speed zones (40, 50, 60 and 100km/h), beliefs about the risks and enforcement of speeding behaviour as well as technology to reduce speeding. RESULTS: Almost half of the sample (47%) reported exceeding the speed limit in 100km/h zones, although only a small number of these drivers (<0.5%) did so by 11km/h or more. Age and sex were related to speed limit non-compliance. Males were more likely to be classified as mid-level speeders, defined as being up to 10km/h over the limit, and excessive speeders (11+km/h over the limit). Younger drivers were also more likely to be non-compliant. When compared to compliant drivers, non-compliers perceived less risk of a serious crash, reported greater likelihood of exceeding the speed limit when they believed they would not be detected, and reported a higher level of social acceptability of speeding. Only one-third of the sample reported prior knowledge of intelligent speed assist (ISA) technology, however, once explained, the majority agreed it would be personally useful (64%). Speed non-compliers were somewhat less likely to support the usefulness of ISA than speed limit compliant drivers. CONCLUSION: These findings can be used to target appropriate interventions and road safety messages, aimed at reducing speeding behaviour. Measures designed to address perceived social acceptability of speed behaviour, the increased crash risk associated with speeding, and the threat of detection are recommended.


Assuntos
Condução de Veículo/psicologia , Autorrelato , Acidentes de Trânsito/prevenção & controle , Acidentes de Trânsito/psicologia , Adolescente , Adulto , Fatores Etários , Idoso , Atitude , Austrália , Condução de Veículo/estatística & dados numéricos , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Assunção de Riscos , Fatores Sexuais , Adulto Jovem
9.
Accid Anal Prev ; 102: 202-212, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-28324820

RESUMO

Motorcycle riders are over-represented in road fatalities in Australia. While riders represent 18% of the road users killed each year, motorcycle registrations constitute only 4.5% of the registered vehicle fleet. The Motorcycle Rider Behaviour Questionnaire (MRBQ) was developed with a view toward understanding behaviours likely to be associated with crash risk. These include behaviours that are either intentional (such as violations of road and speed regulations and stunts) or unintentional (such as errors relating to traffic or control of the motorcycle), as well as protective behaviours related to use of safety equipment. The dual aims of the current study were, first, to determine the appropriate structure of a modified version of the MRBQ for use in a representative sample of riders in Australia and, second, to understand which MRBQ factors are associated with crash involvement. A stratified sampling procedure was undertaken to ensure the socio-economic status of local government area, age and gender of the sample was representative of the broader population of riders in New South Wales, Australia. The sample consisted of 470 riders (males=89%). Exploratory factor analysis revealed a 29-item, five factor structure was suitable on the Australian data encompassing traffic errors, speed violations, protective gear, control errors and stunts. Overall, riders reported relatively safe behaviours, with frequent use of protective gear and infrequent aberrant behaviours. However, even though infrequent, violations of speed and errors related to control of the motorcycle increased the odds of near-crash involvement, whilst stunt behaviours were associated with increased odds of crash involvement. Interventions and countermeasures need to target these specific behaviours.


Assuntos
Acidentes de Trânsito , Destreza Motora , Motocicletas , Assunção de Riscos , Acidentes de Trânsito/estatística & dados numéricos , Adolescente , Adulto , Austrália/epidemiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Motocicletas/estatística & dados numéricos , New South Wales , Equipamentos de Proteção , Inquéritos e Questionários , Adulto Jovem
10.
Risk Anal ; 37(1): 52-64, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26970284

RESUMO

The current study investigated the validity of the revised (25-item) version of the driving anger expression inventory (DAX) on a novel sample of 385 drivers from Ukraine. The roles of sex and gender in relation to self-reported aggressive tendencies were also examined. Confirmatory factor analysis supported the four-factor structure of the DAX (adaptive/constructive expression; use of the vehicle to express anger; verbal aggressive expression; and personal physical aggressive expression), and the three aggressive factors were found to have positive relationships with trait anger and driving anger, while adaptive/constructive expression was negatively related to trait and driving anger. Drivers who reported recent near-misses or loss of concentration scored higher on verbal aggressive expression. Those who had recently received a traffic ticket also reported higher levels of all three types of aggressive anger expression. Further, the presence of feminine traits, but not sex, predicted more adaptive/constructive behaviors and lower scores for verbal aggressive expression, personal physical aggressive expression, and total aggressive expression. However, masculine traits did not predict any of these factors. This research concludes that the revised DAX is a valid tool to measure the expression of driving anger and that the endorsement of feminine traits is related to less aggressive expression of driving anger.

11.
Accid Anal Prev ; 98: 241-251, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27770690

RESUMO

BACKGROUND: Alcohol contributes to approximately 30% of all serious crashes. While the majority of drivers acknowledge the risks associated with drink-driving, a significant proportion of the population continue to engage in this behaviour. Attitudes towards drink-driving as well as personal alcohol consumption patterns are likely to underpin a driver's decision to drink-drive. These associations were explored in the current study. METHODS: A large (N=2994) cross-sectional online survey of a representative sample of drivers in Australia was conducted. Participants provided information about their own alcohol consumption patterns, drink-driving behaviour as well as attitudes towards drink-driving (own and others) and enforcement strategies. RESULTS: Alcohol consumption patterns differed according to age, gender and work status. Drivers who reported drink-driving behaviour and had high risk alcohol consumption patterns were less likely to agree that drink-driving leads to increased crash risk and more likely to agree they drink and drive when they believed they could get away with it. In contrast, drivers who did not report drink-driving and had low risk consumption patterns were more likely to report that the enforcement strategies are too lenient. Binary logistic regression showed that high risk alcohol consumption patterns and agreement from drivers that they drink and drive when they believe they can get away with it had the strongest associations with drink-driving. These findings highlight the relationships between one's drinking patterns, drink-drive behaviour and attitudes towards drink-driving and drink-driving enforcement CONCLUSIONS AND IMPLICATIONS: The patterns of associations that emerged suggest that drink-driving is the expression of a broader health issue for the most "at-risk" cohort of drinkers. The decision to drink and drive may result from a need borne from an alcohol dependent lifestyle exacerbated by a social acceptability of the behaviour and positive attitudes towards one's ability to drink-drive with few adverse consequences. Therefore, the broader alcohol consumption patterns of drink-drivers needs to be considered when targeting drink-drive reductions.


Assuntos
Acidentes de Trânsito/estatística & dados numéricos , Consumo de Bebidas Alcoólicas/epidemiologia , Condução de Veículo/estatística & dados numéricos , Comportamento Perigoso , Adulto , Idoso , Intoxicação Alcoólica/epidemiologia , Austrália/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Assunção de Riscos , Segurança , Meio Social , Adulto Jovem
12.
Accid Anal Prev ; 86: 186-98, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26584016

RESUMO

The Driver Behaviour Questionnaire (DBQ) is a widely used measure of driving behaviours that may increase a driver's risk of crash involvement. However, there are several different versions of the DBQ varying in terms of number of items and factor structure. The aim of the current research was to assess the construct validity of the popular 28-item four-factor DBQ solution in a representative sample of drivers in Australia. A further aim was to test the factorial invariance of the measure across gender, age and also between fleet and non-fleet drivers using multigroup confirmatory factor analyses. Data on a range of attitudes towards road safety were collected using an online survey. A stratified sampling procedure was undertaken to ensure the age, gender and location distributions of participants were representative of the Australian population. A total of 2771 responses were obtained from fully licensed motor vehicle drivers (male: 46%). Confirmatory factor analysis supported the 28-item four-factor DBQ in the Australian sample. The DBQ was also found to be gender-invariant and strong partial measurement invariance was found for drivers aged from 26 to 64, but not for younger (17-25) or older (65-75) drivers. Modifications to the DBQ suggest how the DBQ can be improved for use in these two age groups.


Assuntos
Condução de Veículo/estatística & dados numéricos , Inquéritos e Questionários , Adolescente , Adulto , Fatores Etários , Idoso , Austrália , Análise Fatorial , Feminino , Humanos , Licenciamento , Masculino , Pessoa de Meia-Idade , Segurança , Fatores Sexuais , Adulto Jovem
13.
Accid Anal Prev ; 88: 20-8, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26710267

RESUMO

Trait driving anger is often, but not always, found to predict both the intensity of anger while driving and subsequent crash-related behaviours. However, a number of studies have not found support for a direct relationship between one's tendency to become angry and anger reported while driving, suggesting that other factors may mediate this relationship. The present self-report study investigated whether, in anger provoking driving situations, the appraisals made by drivers influence the relationship between trait and state anger. A sample of 339 drivers from Ukraine completed the 33-item version of the Driver Anger Scale (DAS; Deffenbacher et al., 1994) and eight questions about their most recent experience of driving anger. A structural equation model found that the intensity of anger experienced was predicted by the negative evaluations of the situation, which was in turn predicted by trait driving anger. However, trait driving anger itself did not predict anger intensity; supporting the hypothesis that evaluations of the driving situation mediate the relationship between trait and state anger. Further, the unique structure of the DAS required to fit the data from the Ukrainian sample, may indicate that the anger inducing situations in Ukraine are different to those of a more developed country. Future research is needed to investigate driving anger in Ukraine in a broader sample and also to confirm the role of the appraisal process in the development of driving anger in both developed and undeveloped countries.


Assuntos
Agressão/psicologia , Ira , Condução de Veículo/psicologia , Adaptação Psicológica , Adolescente , Adulto , Idoso , Atitude , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Personalidade , Autorrelato , Inquéritos e Questionários , Ucrânia , Adulto Jovem
14.
Injury ; 40(3): 253-6, 2009 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19070841

RESUMO

Doctors are frequently asked by patients whether it is safe to drive with an upper limb immobilised in a cast. In the literature there are no objective measurements of the effects of upper-limb immobilisation upon driving performance. Eight healthy volunteers performed four 20-min driving circuits in a driving simulator (STISIM 400W), circuits 1 and 4 without immobilisation and circuits 2 and 3 with immobilisation. Immobilisation involved a lightweight below-elbow cast with the thumb left free. Volunteers were randomised to right or left immobilisation for circuit 2, and the contralateral wrist was immobilised for circuit 3. Circuits included urban and rural environments and specific hazards (pedestrians crossing, vehicles emerging from a concealed entrance, traffic lights changing suddenly, avoidance of an oncoming vehicle in the driver's carriageway). Limb immobilisation led to more cautious rural and urban driving, with less adjustment of speed and lateral road position than when unrestricted. However when responding to hazards immobilisation caused less safe driving, with higher speeds, a greater proximity to the hazard before action was taken and less steering adjustment. The effects of restriction upon performance were more prevalent and severe with right-arm immobilisation. Upper-limb immobilisation appears to have little effect on the ability to drive a car unchallenged, but to adversely affect responses to routine hazards. Advice on ability to drive safely should be cautious, as the impact of immobilisation appears to be more subtle and wide ranging than previously thought.


Assuntos
Condução de Veículo , Imobilização/efeitos adversos , Segurança , Extremidade Superior/lesões , Simulação por Computador , Feminino , Humanos , Masculino , Tempo de Reação , Assunção de Riscos , Análise e Desempenho de Tarefas , Adulto Jovem
15.
Eur J Biochem ; 267(21): 6443-51, 2000 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-11029588

RESUMO

We have used site-directed chemical labelling to demonstrate the membrane topology and to identify neighbouring subunits of subunit 8 (Y8) in yeast mitochondrial ATP synthase (mtATPase). Unique cysteine residues were introduced at the N or C-terminus of Y8 by site-directed mutagenesis. Expression and targeting to mitochondria in vivo of each of these variants in a yeast Y8 null mutant was able to restore activity to an otherwise nonfunctional ATP synthase complex. The position of each introduced cysteine relative to the inner mitochondrial membrane was probed with thiol-specific nonpermeant and permeant reagents in both intact and lysed mitochondria. The data indicate that the N-terminus of Y8 is located in the intermembrane space of mitochondria whereas the C-terminus is located within the mitochondrial matrix. The proximity of Y8 to other proteins of mtATPase was tested using heterobifunctional cross-linking reagents, each with one thiol-specific reactive group and one nonspecific, photoactivatible reactive group. These experiments revealed the proximity of the C-terminal domain of Y8 to subunits d and f, and that of the N-terminal domain to subunit f. It is concluded that Y8 possesses a single transmembrane domain which extends across the inner membrane of intact mitochondria. As subunit d is a likely component of the stator stalk of mitochondrial ATP synthase, we propose, on the basis of the observed cross-links, that Y8 may also be part of the stator stalk.


Assuntos
Cisteína/metabolismo , Mitocôndrias/enzimologia , Engenharia de Proteínas , ATPases Translocadoras de Prótons/química , ATPases Translocadoras de Prótons/metabolismo , Leveduras/enzimologia , Sequência de Aminoácidos , Sítios de Ligação , Western Blotting , Respiração Celular/efeitos dos fármacos , Reagentes de Ligações Cruzadas , Cisteína/genética , Dissulfetos/metabolismo , Inibidores Enzimáticos/farmacologia , Proteínas Fúngicas/química , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Proteínas de Membrana/química , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Mersalil/farmacologia , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Modelos Moleculares , Mutação/genética , Ligação Proteica , Estrutura Quaternária de Proteína/efeitos dos fármacos , Subunidades Proteicas , ATPases Translocadoras de Prótons/genética , Leveduras/citologia , Leveduras/efeitos dos fármacos , Leveduras/metabolismo
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